A Plaque Assay for the Simian Rotavirus SA11
نویسندگان
چکیده
منابع مشابه
A plaque assay for the simian rotavirus SAII.
A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SAII is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SAII produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.
متن کاملPrimary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence.
DNA sequences complementary to the double-stranded RNA coding for the neutralization antigen (glycoprotein VP7) of simian rotavirus SA11 have been cloned. The VP7 gene consists of 1,062 nucleotides, containing an uninterrupted coding sequence of 978 nucleotides which specifies a glycoprotein of 326 amino acids. The significance of a second possible initiation site 30 nucleotides downstream from...
متن کاملPreferential Assortment of Rearranged NSP3 Gene in the Genetic Background of Simian Rotavirus SA11
Selective assortment of rotavirus NSP3 gene in the genetic background of simian rotavirus strain SA11 was analyzed by using single-NSP3 gene-substitution reassortants having NSP3 gene from human rotavirus strain KU (KU-R) or porcine rotavirus strain OSU (OSU-R) in the SA11 background, and an SA11-derived variant Ga613 having a rearranged SA11NSP3 gene which is 937 nucleotides longer than the or...
متن کاملEffect of actinomycin D on simian rotavirus (SA11) replication in cell culture.
Rotaviruses are the major cause of viral diarrhea in humans and animals. Actinomycin D (Act D) is an antibiotic that intercalates DNA and therefore inhibits DNA-dependent transcription. The current study was carried out to assess the influence of Act D on the replication of simian rotavirus (SA11) in cell culture. Virus-infected MA-104 cell cultures were studied in the presence of Act D at conc...
متن کاملDerivation of neutralizing monoclonal antibodies against rotavirus.
Monoclonal antibodies were derived against the SA11 simian, NIC bovine, and Wa human rotavirus strains and characterized by enzyme-linked immunosorbent assay, plaque neutralization, and hemagglutination inhibition. Several strain SA11-specific antibodies were found to have neutralizing and hemagglutination-inhibiting capacity.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1979
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-43-3-513